We are fascinated by the amazing complexity and myriad functions of glycoconjugates in human health and disease. Still more enthralling are the intricate membrane-associated pathways that lead to the cellular biogenesis of these important macromolecules.
Our group applies approaches and technologies from a wide range of synergistic fields including chemical biology (for inhibitor and probe development), biochemistry and biophysics (for analyses within and beyond native and model membranes), and cellular, molecular and microbiology to unravel these pathways.
Ultimately we seek to decipher the molecular logic of glycoconjugate biosynthesis and to identify processes to target in the study of infectious disease.
Monotopic PGTs set sail on the membrane
Nature Chemical Biology 2018, 14, 538-541.
"Membrane association of monotopic phosphoglycosyl transferase underpins function"
Leah C. Ray, Debasis Das, Sonya Entova, Vinita Lukose, Andrew J. Lynch, Barbara Imperiali& Karen N. Allen doi: 10.1038/s41589-018-0054-z
SEE ALSO: https://cen.acs.org/analytical-chemistry/structural-biology/Enzyme-catalyzes-cross-membrane-reaction/96/i22
All you ever needed to know about phosphoglycosyl transferases!
"Bacterial Phosphoglycosyl Transferases: Initiators of Glycan Biosynthesis at the Membrane Interface" Lukose, V.; Walvoort, M. T. C.; Imperiali, B. Glycobiology. 2017, 27, 820-833.
"Analysis of a Dual Domain Phosphoglycosyl Transferase Reveals a Ping-Pong Mechanism with a Covalent Enzyme Intermediate", Das, D.; Kuzmic, P.; Imperiali, B. Proc. Natl. Acad. Sci. U.S.A. 2017, 114(27):7019-7024. PMCID: PMC5502628.
Same but different
“Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases“ Eichler, J.; Imperiali, B. Trends Biochem. Sci. 2018, 43, 9-16.